The game features two different modes of play - 'Story mode' and 'Score mode'. In story mode the game follows fifteen days of Rayman's imprisonment by the Rabbids. Each day, Rayman must complete at least three trials, followed by one special "boss trial", such as a first-person rail shooter using plungers, or a racing game in which the player controls a warthog and uses a flyswatter as a riding crop. Completing trials earns Rayman various costumes and matching music, including Gangsta, Raymaninho (a portmanteau of the title character's name and football star Ronaldinho), Disco, Gothic, Caramba, Rock'n'Roll, Granny, DeeJay, and Bunny. Trial completion also earns plungers and after accumulating enough, Rayman builds a ladder up the edge of his jail cell and escapes to freedom. In Score mode, the player can repeat past trials in an attempt to improve their score or can play the game as a multiplayer party game.
Minigames fall into one of four categories: Bunny Hunt, Sports, Challenges, and 'Shake your Booty!' dancing. Bunny Hunt comprises first-person rail shooter stages, all appearing in Story Mode as "boss" stages that the player may play for Score, Time, or Survival with the goal of obtaining the highest score possible with only one life. Two players can participate in Co-op Bunny Hunt, but Survival is not available for multiplayer. The Sports minigames can be played for Workout, which requires rapid movement of the Wii Remote, and Precision, which involves the controls of the remote. Sports minigames also include 'Get Going!' racing stages, including four warthog racing games and a skydiving race. Challenges are various games found in Score Mode which must be played one after the other with the goal of a high combined score in a Triathlon, Pentathlon, or Decathlon. The 'Shake your Booty!' category involves dance-themed minigames and are found in Story Mode each day. Also, there are "Skill" minigames that do not fall under any of the other categories. Variations of the mini-games are featured in the DS version. Games such as "Feed the bunnies", "Ghost hunt", "Bunny invasion", "Stop the mines" and "Super Rabbid" are used. The stylus is used for all of the minigames, unlike the rest of the gameplay.
The Game Boy Advance and Nintendo DS versions of the game are, unlike their console counterparts, traditional platformers. In the Game Boy Advance version, the costumes that Rayman acquires give him special abilities, such as using the Granny outfit to sneak past cameras, or the DeeJay outfit to break open vents. The Nintendo DS version allows players to use the stylus to perform certain actions, such as controlling wind or setting rabbits on fire.